ACUTE TOXICITY TO DAPHNIA MAGNA/ FeMn slag (Ferro-manganese slag)/ OECD 202 / GLP
Introduction. A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute lmmobilisation Test", referenced as Method 02 of Commission Regulation (EC) No. 440/2008.
Results. The 48-Hour EL*50 for the test item to Daphnia magna based on nominal loading rates was 43 mg/l loading rate WAF with 95% confidence limits of 41 - 46 mg/l loading rate WAF. The No Observed Effect Loading rate was 32 mg/l loading rate WAF.
The test item contains manganese. The test concentrations were monitored by analysis of the test samples for manganese only.
Analysis of the fresh media at 0 hours showed measured concentrations of manganese to range from 1.13 to 3.79 mg/l. Analysis of the old media at 48 hours showed measured concentrations of manganese to range from 1.16 to 3.62 mg/l.
The dissolved test item may have been one or several components of the test item. Given that toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
The 48-Hour EC50 for the reference item to Daphnia magna based on nominal concentrations was 0.78 mg/l with 95% confidence limits of 0.58 - 0.72 mg/l. The No Observed Effect Concentration was 0.32 mg/l.
*EL = Effective Loading rate
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