ACUTE TOXICITY TO DAPHNIA MAGNA/ Mn metal (Manganese metal)/ OECD 202 / GLP
Introduction. A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test", referenced as Method C2 of Commission Regulation (EC) No. 440/2008.
Results. The 48-Hour EC50 for the test item to Daphnia magna based on nominal test concentrations was greater than 100% v/v saturated solution and correspondingly the No Observed Effect Concentration was 100% v/v saturated solution.
Analysis of the fresh test preparations at 0 hours showed measured concentrations of 1.60 and 1.62 mg/l as a test item. Analysis of the old media at 48 hours showed measured concentrations of 1.54 and 1.59 mg/l as a test item.
Given that no decline in measured concentration was observed over the test period, the results are based on the mean measured test concentration as a test item only. This was calculated to be 1.6 mg/l.
The 48-Hour EC50 for the test item to Daphnia magna, based on the mean measured test concentration as a test item, was greater than 1.6 mg/l and correspondingly the No Observed Effect Concentration was 1.6 mg/l.
This study showed that there were no toxic effects at saturation.
The 48-Hour EC50 for the reference item to Daphnia magna based on nominal concentrations was 0.65 mg/I with 95% confidence limits of 0.58 - 0.72 mg/l. The No Observed Effect concentration was 0.32 mg/l.
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