ACUTE TOXICITY TO DAPHNIA MAGNA/ Sinter ore (Manganese ores, reduced)/ OECD 440/2008/ GLP
Introduction. A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed that described in the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute. Immobilisation Test" referenced as Method C2 of Commission Regulation (EC) No. 440/2008.
Results. The 48-Hour EL*50 for the test item to Daphnia magna based on nominal loading rates was greater than 100 mg/l loading rate WAF and correspondingly the No Observed Effect Loading rate was 100 mg/l loading rate WAF.
It was considered unnecessary and unrealistic to test at loading rates in excess of 100 mg/l.
The test preparations were analysed for manganese content. Analysis of the test preparations at 0 hours showed measured concentrations of manganese to be 0.330 and 0.326 mg/l. Analysis of the old media at 48 hours showed measured test concentrations of manganese to be 0.290 and 0.298 mg/l.
The dissolved test item may have been one or several components of the test item.
Given that toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
The 48-Hour EC50 for the reference item to Daphnia magna based on nominal concentrations was 0.65 mg/l with 95% confidence limits of 0.58 - 0.72 mg/l. The No Observed Effect Concentration was 0.32 mg/l.
*EL = Effective Loading rate
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