ACUTE TOXICITY TO RAINBOW TROUT / Mn3O4 (Trimanganese tetraoxide)/ OECD 203/ GLP
Introduction. A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method C1 of Commission Regulation (EC) No. 440/2008.
Results. The 96-Hour LC50 based on nominal test concentrations was greater than 100% v/v saturated solution and correspondingly the No Observed Effect Concentration was 100% v/v saturated solution.
The test item contained a theoretical manganese content of 72% w/w. The test samples were analysed for manganese only. Analysis of the test preparations at 0, 24 and 96 hours showed measured concentrations of manganese to be less than the limit of quantitation (LOQ) of the analytical method (assessed as 0.050 mg manganese/l).
This study showed that there were no toxic effects at saturation.
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