ACUTE TOXICITY TO RAINBOW TROUT(Oncorhynchus mykiss)/MnS(Manganese Sulphide)/OECD 203/ GLP
Introduction. A study was performed to assess the acute toxicity of the test item to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD Guidelines for Testing of Chemicals (1992) No 203, "Fish, Acute Toxicity Test" referenced as Method 0.1 of Commission Regulation (EC) No. 440/2008.
Results. The 96-Hour LC50 based on nominal test concentrations was greater than 100% v/v saturated solution. The No Observed Effect Concentration was 56% v/v saturated solution.
The test item contained a theoretical manganese content of 63% w/w. The test samples were analysed for manganese only. Analysis of the test preparations at 0, 24 and 96 hours showed measured concentrations to range from 1.25 to 14.7 mg/l as test item.
Given that no decline in measured concentration was observed over the test period, the results are based on the mean measured test concentrations as test item only. These were calculated to be 1.3, 2.3, 4.0, 7.8 and 13 mg/l.
The 96-Hour LC50 based on mean measured test concentrations was greater than 13 mg/l. The No Observed Effect Concentration was 7.8 mg/l.
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