DAPHNID,CERIODAPHNIA DUBIASURVIVALAND REPRODUCTION TEST/FeMn slag(Ferro-manganese slag)/OECD 211/GLP
Introduction. A study was performed to assess the effect of the test item on the survival and reproduction of Ceriodaphnia dubia over an 8-Day period. The method followed that described in the OECD Guidelines for Testing of Chemicals No 211 (1998), "Daphnia magna, Reproduction Test", US EPA Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (Method 1002.0), and OPPTS Ecological Effects Test Guideline, Daphnid Chronic Toxicity Test OPPTS 850.1300.
Results. The 8-Day EL*50 (immobilisation) value, based on nominal loading rates, for the parental Ceriodaphnia generation (P1) was calculated to be 57 mg/l loading rate WAF with 95% confidence limits of 32 - 100 mg/l loading rate WAF.
The 8-Day EL*50 (reproduction) value based on nominal loading rates was calculated to be 11 mg/l loading rate WAF with 95% confidence limits of 6.5 - 20 mg/l loading rate WAF.
The "Lowest Observed Effect Loading Rate" was considered to be 10 mg/l loading rate WAF on the basis that at this loading rate significantly fewer live young per adult (P<0.05) were produced when compared to the control.
*EL = Effective Loading rate
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