DAPHNID, CERIODAPHNIA DUBIA SURVIVAL AND REPRODUCTION TEST/ Mn metal (Manganese metal)/OECD 211/GLP
Introduction. A study was performed to assess the effect of the test item on the survival and reproduction of Ceriodaphnia dubia over an 8-Day period. The method followed that described in the OECD Guidelines for Testing of Chemicals No 211 (1998), "Daphnia magna, Reproduction Test", US EPA Short-term Methods for Estimating the Chronic Toxicity of Effluents and Receiving Waters to Freshwater Organisms (Method 1002.0), and OPPTS Ecological Effects Test Guideline (Daphnid Chronic Toxicity Test OPPTS 850.1300).
Results. Analysis of the fresh test preparations on Days 0, 3, 5 and 7 showed measured concentrations to range from 0.353 to 8.15 mg/l as the test item. Analysis of the old media on Days 3, 5, 7 and 8 showed measured concentrations to range from 0.369 to 8.36 mg/l as the test item.
Given that no decline in measured concentration was observed over each media renewal period, the results were based on the mean measured test concentration as a test item only. These were calculated to be 0.52, 1.7 and 5.1 mg/l.
The 8-Day EC50 (immobilisation) value based on the mean measured concentrations as a test item for the parental Ceriodaphnia generation (P1) was calculated to be 5.1 mg/l.
The 8-Day EC50 (reproduction) value based on the mean measured concentrations as a test item was calculated to be 3.6 mg/l with 95% confidence limits of 2.0 - 13 mg/l.
The "Lowest Observed Effect Concentration" was considered to be 5.1 mg/l as a test item on the basis that at this test concentration significantly fewer live young per adult (P<0.05) were produced when compared to the control.
The "No Observed Effect Concentration" was considered to be 1.7 mg/l on the basis that at this test concentration there were no significant mortalities (immobilisation) observed in the parental generation (P1) and that there were no significant differences (P≥0.05) between the control and the 1.7 mg/l test group in terms of numbers of live young produced per adult by Day 8.
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