THE INHIBITORY EFFECT ON THE RESPIRATION/ Mn3O4 (Trimanganese tetraoxide)/ OECD 209 / GLP
Introduction. A study was performed to assess the effect of the test item on the respiration of activated sewage sludge. The method followed that described in the OECD Guidelines for Testing of Chemicals (1984) No 209 "Activated Sludge, Respiration Inhibition Test", Method 0.11 of Commission Regulation (EC) No. 440/2008 and US EPA Draft Ecological Effects Test Guidelines OPPTS 850.6800.
Results. The effect of the test item on the respiration of activated sewage sludge gave a 3-Hour EC50 of greater than 1000 mg/l. The No Observed Effect Concentration (NOEC) after 3 hours exposure was 1000 mg/l.
It was considered unnecessary and unrealistic to test at concentrations in excess of 1000 mg/l.
The reference item gave a 3-Hour EC50 value of 8.3 mg/l, 95% confidence limits 6.6 - 10 mg/l.
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